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Fig. 1 | Parasites & Vectors

Fig. 1

From: CD3+CD4negCD8neg (double negative) T lymphocytes and NKT cells as the main cytotoxic-related-CD107a+ cells in lesions of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis

Fig. 1

Frequencies of NK and NKT cells; CD4+, CD8+, double positive (DP) and double negative (DN) CD3+ T-lymphocyte subpopulations. a-f Flow cytometry-representative protocol: Cells obtained from cutaneous leishmaniasis lesions were stained with anti-CD3, anti-CD4, anti-CD8, anti-CD56, anti-CD107a and 7-AAD. Lymphocytes region was created on forward (FSC) vs side (SSC) scatter dot-plot (a). Cells gated on [Lymphs.1] were analyzed through dot-plots SSC-Height vs SSC-Area (b) and SSC-Height vs SSC-Width (c) to exclude doublets and debris. d Dead cells (7-AAD+) were excluded from the analysis and a gate encompassing 7-AADneg cells was performed. e Based on this gate, NK cells (CD56+CD3neg), NKT (CD56+CD3+) and T lymphocytes (CD56negCD3+) were identified. f Based on T lymphocyte gate (T Lymph), CD4, CD8, double-negative (DN) and double-positive (DP) T lymphocytes were determined. g Bar graphs representing the mean ± SEM of percentages of NKT and NK cells; CD4+, DP, CD8+ and DN T lymphocytes obtained from cutaneous leishmaniasis lesions of 18 patients. Statistical analyses were performed using ANOVA test and Dunn’s post-hoc test. Results were considered significant with P < 0.05 (*P < 0.05; **P < 0.01; ***P < 0.001)

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