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Fig. 4 | Parasites & Vectors

Fig. 4

From: CD3+CD4negCD8neg (double negative) T lymphocytes and NKT cells as the main cytotoxic-related-CD107a+ cells in lesions of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis

Fig. 4

Correlation analysis of granzyme B, IFN-γ and TNF-α production, frequency of CD107+ cells and lesion size from lesions of CL patients (n = 10). Granzyme B, IFN-γ and TNF-α levels were quantified in culture media from macerated lesions biopsied from ten patients. All samples were prepared following CBA multiplex kit manual and the cytokines were detected within a range of 10–2500 pg/ml. We observed positive correlations between frequency of CD107a+ cells and granzyme B production (a); lesion size and granzyme B production (b); lesion size and frequency of CD107a+ cells in CL lesions (c); lesion size and IFN-γ production (d); and lesion size and TNF-α production (e). The central line represents median values and the graph show best fitted lines with 95% confidence interval. Statistical analyses were performed using Spearman’s test (r: correlation coefficient). Results were considered significant with P < 0.05. Each point represents one CL patient

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