Fig. 2

Percent difference between raw qPCR and Kato-Katz measurements and average of four technical replicates, versus the average measurement. a For samples A-D, the percent difference between each measurement relative to the average of four technical replicates (wells on the same qPCR plate from the same extraction) was calculated. This was plotted against the average of the four measurements. The percent difference from the mean of each of four readings was also plotted against the mean for 284 stool samples tested by qPCR during the screening phase where at least one reading was positive for A. lumbricoides (shown in black). b The percent difference of each raw read from the mean of four reads from each slide was plotted against the mean of the four egg counts. Though each of these slides was read by five readers, only four were analyzed here, in order to be as comparable as possible to the four technical replicates analyzed in (a). The scale of the X-axis was chosen to represent a similar range of egg intensities as seen in (a)