Skip to main content

Advertisement

Table 5 Mean and variance calculated from four technical replicate measurements from each of 16 slides

From: Sources of variability in the measurement of Ascaris lumbricoides infection intensity by Kato-Katz and qPCR

Sample number qPCR KK qPCR KK CoV KK/CoV qPCRa
Mean Variance Mean Variance Coefficient of variation
1 0.1300 0.00035684 375 10,061 0.15 0.27 2
2 0.0004 0.00000003 8 78 0.46 1.18 3
3 0.0285 0.00000085 116 425 0.03 0.18 5
4 0.0285 0.00000085 117 140 0.03 0.10 3
5 0.0019 0.00000005 3 5 0.12 0.68 6
6 0.0067 0.00000060 76 418 0.12 0.27 2
7 0.1300 0.00035684 313 13,847 0.15 0.38 3
8 0.0149 0.00000174 140 44 0.09 0.05 1
9 0.0011 0.00000004 12 108 0.17 0.88 5
10 0.0149 0.00000174 210 341 0.09 0.09 1
11 0.0606 0.00019056 94 582 0.23 0.26 1
12 0.0634 0.00019056 104 246 0.22 0.15 1
13 0.0634 0.00000766 95 475 0.04 0.23 5
14 0.0634 0.00000766 114 2025 0.04 0.39 9
15 0.0835 0.00002502 123 1294 0.06 0.29 5
16 0.0835 0.00002502 97 1348 0.06 0.38 6
  1. Since some of the KK slides used in this analysis were prepared from the same stool (slides A and B), but each stool was only analyzed once by qPCR, some mean and variance qPCR measurements are shown in duplicate in this chart. The subset of samples used for this analysis is described in Table 2
  2. a Coefficient of variation (variance divided by mean) of Kato-Katz measurements divided by the coefficient of variation of measurements from the same stool