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Table 1 Results of samples collected at Moredun, Scotland and Ghent, Belgium and tested by three different methods; multiplexed-tandem (MT)-PCR eggs (MT-PCR(e)), MT-PCR larvae (MT-PCR(l)) and larval culture (LC)

From: Multiplexed-tandem PCR for the specific diagnosis of gastrointestinal nematode infections in sheep: an European validation study

    MT-PCR gene copy number (calculated percentage)
Sample id (location) EPG Test Tel Haem Trich Cho Oeso Coc
M1 585 Mt-PCR(e) a 693 (21) 0 (0) 2,572 (79)* 0 (0) 0 (0) 0 (0)
(Moredun)   MT-PCR(l) a 0 (0) 0 (0) 120 (100)* 0 (0) 0 (0) 0 (0)
   LC % 83* 0 17 0 0
M2 90 Mt-PCR(e) a 458 (94) 0 (0) 0 (0) 0 (0) 31 (6) 0 (0)
(Moredun)   MT-PCR(l) a 2,062 (53) 0 (0) 45 (1) 1,788 (45) 44 (1) 0 (0)
   LC % 85 0 0 15 0
M3 243 Mt-PCR(e) a 165 (29) 0 (0) 379 (66) 0 (0) 29 (5) 0 (0)
(Moredun)   MT-PCR(l) a 261 (18) 0 (0) 1,123 (79) 0 (0) 43 (3) 0 (0)
   LC % 38 0 58 4 0
M4 30 Mt-PCR(e) a 24 (3) 0 (0) 49 (6) 0 (0) 772 (91) 0 (0)
(Moredun)   MT-PCR(l) a 0 (0) 0 (0) 295 (14) 0 (0) 1,799 (86) 0 (0)
   LC % 6 0 12 82 0
M5 176 Mt-PCR(e) a 1,997 (86)* 0 (0) 26 (1) 309 (13) 0 (0) 0 (0)
(Moredun)   MT-PCR(l) a 0 (0) 0 (0) 33 (1) 2,503 (99)* 0 (0) 0 (0)
   LC % 7 0 0 93* 0
M6 59 Mt-PCR(e) b 0 (0) 0 (0) 146 (11) 1,241 (89) 0 (0) 0 (0)
(Moredun)   MT-PCR(l) a 77 (0.3) 0 (0) 325 (1) 22,566 (99) 0 (0) 0 (0)
   LC % 4 0 1 94 0
M7 13 Mt-PCR(e) b 113 (100) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0)
(Moredun)   MT-PCR(l) a 631 (91) 0 (0) 61 (9) 0 (0) 0 (0) 0 (0)
   LC % 100 0 0 0 0
M8 11 Mt-PCR (e) 16,693 (100) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0)
(Moredun)   MT-PCR(l) b 514 (76) 0 (0) 0 165 (24) 0 (0) 0 (0)
   LC % 97 0 0 3 0
M9 171 Mt-PCR(e) b 3,994 (82) 0 (0) 602 (12) 133 (3) 158 (3) 0 (0)
(Moredun)   MT-PCR(l) a 1,075 (79) 0 (0) 245 (18) 0 (0) 42 (3) 0 (0)
   LC % 79 0 15 6 0
M10 36 Mt-PCR (e) 33,346 (30) 0 (0) 47,858 (43)** 24,912 (22) 2,709 (3) 2,100 (2)
(Moredun)   MT-PCR(l) 19 (0.03) 0 (0) 8,189 (14) 42,319 (72)* 0 (0) 8,100 (14)
   LC % 34 0 2 14 50*
M11 720 Mt-PCR (e) b 358 (1) 27,983 (92) 130 (0.43) 1,792 (6) 253 (1) 0 (0)
(Moredun)   MT-PCR(l) b 0 (0) 4,309 (99) 62 (1) 0 (0) 0 (0) 0 (0)
   LC % 0 (0) 98 0 1 1
B1 176 Mt-PCR(e) 10,762 (85) 15 (0.12) 0 (0) 0 (0) 0 (0) 1,900 (15)
(Ghent)   MT-PCR(l) 14,617 (78) 40 (0.21) 0 (0) 0 (0) 0 (0) 4,000 (21)
   LC % 92 0 8 0 0 0
B2 2 Mt-PCR(e) 13,157 (8) 135,922 (84) 1,793 (1) 11,398 (7) 0 (0) 0 (0)
(Ghent)   MT-PCR(l) 1,619 (1) 79,775 (67) 887 (1) 36,547 (31) 0 (0) 0 (0)
   LC % 10 68 5 17 0
B3 176 Mt-PCR(e) 8,262 (12) 34,497 (51)* 25,297 (37) 0 (0) 0 (0) 0 (0)
(Ghent)   MT-PCR(l) 3,887 (84)* 0 (0) 752 (16) 0 (0) 0 (0) 0 (0)
   LC % 99* 1 0 0 0
B4 72 Mt-PCR(e) 6,914 (71)* 406 (4) 16 (0.16) 2,436 (25) 0 (0) 0 (0)
(Ghent)   MT-PCR(l) 4,154 (13) 21,880 (68)* 2,275 (7) 0 (0) 0 (0) 3,900 (12)
   LC % 17 83* 0 0 0
B5 200 Mt-PCR(e) 22,039 (100) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0)
(Ghent)   MT-PCR(l) 172,336 (100) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0)
   LC % 68 0 32 0 0
B6 986 Mt-PCR(e) 6,741 (5) 39,499 (30) 220 (0.17) 83,044 (63)* 0 (0) 3,100 (2)
(Ghent)   MT-PCR(l) 175 (26) 494 (74)* 0 (0) 0 (0) 0 (0) 0 (0)
   LC % 17 74* 6 0 3
B7 1584 Mt-PCR(e) 153,756 (20) 531,837 (70) 0 (0) 72,565 (10) 945 (0.12) 0 (0)
(Ghent)   MT-PCR(l) 1,195,981 (10) 9,266,891 (77) 1,955 (0.02) 1,436,634 (12) 83,435 (1) 5,900 (0.05)
   LC % 30 65 0 5 0
B8 804 Mt-PCR(e) 267,862 (11) 39 (0.00) 566,001 (24) 0 (0) 1,414,909 (59)* 156,200 (6)
(Ghent)   MT-PCR(l) 8,309 (3) 56 (0.02) 84,321 (28) 0 (0) 28,087 (9) 180,300 (60)*
   LC % 24 1 4 10 61*
  1. aSamples tested at 1/50 dilution
  2. bSamples tested at 1/100 dilution
  3. *Samples with discrepant results
  4. For every sample, the microscopy results are given as eggs per gram of faeces (EPG). The species/genera-specific results of the MT-PCR for T. circumcincta (Tel), Haemonchus spp. (Haem), Trichostrongylus spp. (Trich), C. ovina (Cho), Oesophagostomum spp. (Oeso) and Co. curticei (Coc) are shown as gene copy number or as calculated percentage (%). Discrepancies between the results of the three diagnostic methods are indicated by an asterisk