Fig. 5

High resolution, 3-dimentional, FIB-SEM images of Trypanosoma sp. AAT small epimastigote DNA containing structures. Images were constructed using segmentation. a Segmented mitochondrion (pink), kinetoplast pocket containing kDNA (red), and nucleus (blue) shown with image collected from the electron beam to indicate the position of the organelles inside the cell. b 3D model of DNA containing structures: mitochondrion (pink), kinetoplast pocket (red), and nucleus (blue). c 3D model of kinetoplast pocket (red), which can be attached at multiple places to the mitochondrion (white arrows). d Round disc-shaped kinetoplast pocket (red) (Additional file 2). Scale-bars: a, b, 2 μm; c, 1 μm; d, 0.5 μm