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Table 1 List of primers and gene markers used in this study as MLST scheme

From: Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia

Target gene

Enzyme entry

Chromosomal location

Gene length (bp)

Amplicon size (bp)

Primer sequence

Reference

Glucose-6-phosphate dehydrogenase (g6pdh)

EC 1.1.1.49

20 and 34aa

1,686

881

Fw: ATGGAAGCGTGTGATCGAAT

Rv: GGCTCAACACACTTCAGCAA

[16]

Phosphoglucomutase (pgm)

EC 5.4.2.2

21

1,770

529

Fw: CAGAGAAGCTGACGTCCCAG

Rv: GACGGGTTCACGAAGAAGCG

[21]

Mannose phosphate isomerase (mpi)

EC 5.3.1.8

32

1,287

681

Fw: GGCAAGATGTATGCGGAGTT

Rv: CTCCCCAGGAACCATCTGTA

[16]

Alanine aminotransferase (alat)

EC 2.6.1.21

12

1,494

589

Fw: GTGTGCATCAACCCMGGGAA

Rv: CGTTCAGCTCCTCGTTCCGC

[21]

Aspartate aminotransferase (asat)

EC 2.6.1.1

24

1,169

684

Fw: ACGAGCGCCGTCCGYAA

Rv: TTCCYMCATCCACCAAGC

[15]

Isocitrate dehydrogenase (icd)

EC 1.1.1.42

33

1,278

1,022

Fw: GAATCGGGAAGGAGATCACA

Rv: CATCATAGCCCCAGAGAGGA

[16]

Cytochrome b (cytb)

 

Maxicircle

 

618

Fw: AGCGGAGAGRARAGAAAAGG

Rv: GYTCRCAATAAAATGCAAATC

 
  1. a g6pdh gene is present on chromosomes 20 and 34a only in L. (V) braziliensis; it is present on chromosome 20 in L. (V) panamensis uniquely