Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania ( Viannia ) braziliensis and Leishmania ( Viannia ) panamensis in Colombia

Table 2 Genetic diversity indexes of L. panamensis and L. braziliensis isolates used in this study

Species	Marker	N	S	Eta	Hd	π^a	θ^b	dN/dS
L. panamensis	pgm	105	10	7	0.234	0.00245	0.00422	0.1454
	asat	105	6	6	0.145	0.00057	0.00322	0.1745
	g6pdh	105	6	6	0.180	0.00078	0.00211	0.1276
	mpi	105	7	8	0.113	0.04292	0.05469	0.2346
	icd	105	10	8	0.111	0.00067	0.00422	0.0124
	alat	105	11	10	0.234	0.0066	0.00722	0.2345
	cytb	105	80	85	0.123	0.00304	0.03981	0.2371
L. braziliensis	pgm	58	13	10	0.324	0.02343	0.04123	0.1352
	asat	58	11	7	0.413	0.01509	0.03086	0.1142
	g6pdh	58	15	11	0.319	0.01008	0.02003	0.1174
	mpi	58	21	13	0.496	0.06271	0.06667	0.1376
	icd	58	14	10	0.331	0.02519	0.03113	0.1238
	alat	58	17	9	0.223	0.03412	0.04126	0.1927
	cytb	58	5	5	0.390	0.00104	0.00243	0.1282

Abbreviations: N number of sequences, S number of polymorphic sites, Eta total number of mutations, Hd haplotype diversity
^aπ is an index of nucleotide diversity. This measure is defined as the average number of nucleotide differences per site between any two DNA sequences chosen randomly from the sample population
^bThe mutation parameter (θ) is defined as 4 Nm for autosomal loci of diploid organisms, where N is the effective population size (diploid individuals) and m is the neutral mutation rate (per gene or per base pair) per generation