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Fig. 5 | Parasites & Vectors

Fig. 5

From: Clonorchis sinensis granulin: identification, immunolocalization, and function in promoting the metastasis of cholangiocarcinoma and hepatocellular carcinoma

Fig. 5

Successful construction of stable CsGRN-overexpressed hepatoma cells and cholangiocarcinoma cells. a Green fluorescence was observed under an inverted microscope and was emitted by PLC and RBE cells transformed with pGFP-C1 and pGFP-C1-CsGRN, respectively. The images were magnified at 100× (scale-bar: 200 μm).b Cellular viability of transfected PLC and RBE cells (24 h, 48 h and 72 h after transfection) was detected by Annexin V-APC/7AAD double staining with FACS analysis. c qRT-PCR was used to detect the expression of CsGRN mRNA between the experimental group and control group. d Western blot analysis was used to determine the CsGRN protein expression between the experimental group and control group. *P < 0.05, ***P < 0.001 compared with a matched group

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Parasites & Vectors

ISSN: 1756-3305

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