Fig. 1

Alignment of heterozygous ITS1 sequences of D. immitis from Portuguese canine samples. The haplotypes were inferred based on circulating haplotypes, considering the most parsimonious hypothesis that at least one haplotype is the same as the most common in circulation in the population. The first position on the alignment corresponds to position 604 of the first sequence, AF217800, reversed. The nucleotide codes K, R, S, and W, correspond, respectively to T/G, A/G, G/C and A/T