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Table 2 Tm comparison of L. (L.) amazonensis and L. (L.) infantum amplicons showing a single HRM peak

From: The use of kDNA minicircle subclass relative abundance to differentiate between Leishmania (L.) infantum and Leishmania (L.) amazonensis

 

Number of replicates

Tm Mean ± SD (SE)

D’Agostino & Pearson omnibus normality test

Unpaired t-test with Welch’s correction

K2

P-value

P-value

L. (L.) amazonensis

16

83.91 ± 0.20 (0.05)

0.1456

0.93

< 0.001

L. (L.) infantum

114

84.44 ± 0.36 (0.03)

1.28

0.53

  1. Abbreviations: T m high resolution melting temperature, SD standard deviation, SE standard error