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Table 3 qPCR-ML and qPCR-ama results

From: The use of kDNA minicircle subclass relative abundance to differentiate between Leishmania (L.) infantum and Leishmania (L.) amazonensis

Species qPCR ML (Cq ± SD) qPCR-ama (Cq ± SD)
  Undiluted Undiluted 1:10 1:100 1:1,000 1:10,000
L. (L.) infantum MHOM/TN/80/IPT1 17.26 ± 0.19 negative negative negative nt nt
L. (L.) infantum MHOM/FR/78/LEM75 15.30 ± 0.41 nt 33.80 ± 0.33 37.92 ± 2.23 negative negative
L. (L.) infantum MHOM/ES/81/BCN1 15.80 ± 0.77 33.70 ± 0.33 38.01 ± 1.36 negative negative nt
L. (L.) infantum MHOM/DZ/82/LIPA59 16.43 ± 0.47 33.03 ± 0.61 nt nt negative negative
L. (L.) infantum MHOM/IT/93/ISS822 14.73 ± 0.42 33.89 ± 0.66 nt nt negative negative
L. (L.) infantum MHOM/IT/86/ISS218 15.42 ± 1.03 32.44 ± 1.18 nt nt negative negative
L. (L.) infantum Clinical isolate V2921 17.09 ± 0.12 29.08 ± 0.13 nt nt negativea negative
L. (L.) infantum Clinical isolate 31u 14.30 ± 0.27 28.73 ± 0.78 nt nt negativea negative
L. (L.) infantum Clinical isolate 49u 15.71 ± 0.78 30.02 ± 0.01 nt nt negative negative
L. (L.) infantum Clinical isolate 791 14.88 ± 0.51 27.76 ± 0.07 nt nt 38.81 ± 0.93 negative
L. (L.) infantum Clinical isolate 10816 13.53 ± 0.46 26.53 ± 0.11 nt nt 36.67 ± 2.08 negative
L. (L.) amazonensis MHOM/BR/00/LTB0016 23.79 ± 0.25 17.97 ± 0.40 21.08 ± 0.23 25.00 ± 0.66 27.97 ± 1.21 31.49 ± 0.88
L. (L.) amazonensis IFLA/BR/67/PH8 34.42 ± 0.02 15.32 ± 0.01 19.33 ± 0.32 nt nt nt
L. (L.) amazonensis Clinical isolate 31.66 ± 0.19 20.87 ± 0.24 24.12 ± 0.14 nt nt nt
L. (V.) panamensis Clinical isolate 22.96 ± 0.28 35.57 ± 0.56 nt negative nt nt
L. (V.) guyanensis Clinical isolate 29.71 ± 0.32 33.30 ± 0.17 36.78 ± 0.46 negative nt nt
L. (V.) braziliensis Clinical isolate 28.15 ± 0.85 37.87 ± 0.86 negative negative nt nt
L. (V.) braziliensis MHOM/BR/75/M2904 26.07 ± 1.80 38.19 ± 0.04 negative negative nt nt
  1. Abbreviation: nt not tested
  2. aOne replicate negative