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Table 3 qPCR-ML and qPCR-ama results

From: The use of kDNA minicircle subclass relative abundance to differentiate between Leishmania (L.) infantum and Leishmania (L.) amazonensis

Species

qPCR ML (Cq ± SD)

qPCR-ama (Cq ± SD)

 

Undiluted

Undiluted

1:10

1:100

1:1,000

1:10,000

L. (L.) infantum MHOM/TN/80/IPT1

17.26 ± 0.19

negative

negative

negative

nt

nt

L. (L.) infantum MHOM/FR/78/LEM75

15.30 ± 0.41

nt

33.80 ± 0.33

37.92 ± 2.23

negative

negative

L. (L.) infantum MHOM/ES/81/BCN1

15.80 ± 0.77

33.70 ± 0.33

38.01 ± 1.36

negative

negative

nt

L. (L.) infantum MHOM/DZ/82/LIPA59

16.43 ± 0.47

33.03 ± 0.61

nt

nt

negative

negative

L. (L.) infantum MHOM/IT/93/ISS822

14.73 ± 0.42

33.89 ± 0.66

nt

nt

negative

negative

L. (L.) infantum MHOM/IT/86/ISS218

15.42 ± 1.03

32.44 ± 1.18

nt

nt

negative

negative

L. (L.) infantum Clinical isolate V2921

17.09 ± 0.12

29.08 ± 0.13

nt

nt

negativea

negative

L. (L.) infantum Clinical isolate 31u

14.30 ± 0.27

28.73 ± 0.78

nt

nt

negativea

negative

L. (L.) infantum Clinical isolate 49u

15.71 ± 0.78

30.02 ± 0.01

nt

nt

negative

negative

L. (L.) infantum Clinical isolate 791

14.88 ± 0.51

27.76 ± 0.07

nt

nt

38.81 ± 0.93

negative

L. (L.) infantum Clinical isolate 10816

13.53 ± 0.46

26.53 ± 0.11

nt

nt

36.67 ± 2.08

negative

L. (L.) amazonensis MHOM/BR/00/LTB0016

23.79 ± 0.25

17.97 ± 0.40

21.08 ± 0.23

25.00 ± 0.66

27.97 ± 1.21

31.49 ± 0.88

L. (L.) amazonensis IFLA/BR/67/PH8

34.42 ± 0.02

15.32 ± 0.01

19.33 ± 0.32

nt

nt

nt

L. (L.) amazonensis Clinical isolate

31.66 ± 0.19

20.87 ± 0.24

24.12 ± 0.14

nt

nt

nt

L. (V.) panamensis Clinical isolate

22.96 ± 0.28

35.57 ± 0.56

nt

negative

nt

nt

L. (V.) guyanensis Clinical isolate

29.71 ± 0.32

33.30 ± 0.17

36.78 ± 0.46

negative

nt

nt

L. (V.) braziliensis Clinical isolate

28.15 ± 0.85

37.87 ± 0.86

negative

negative

nt

nt

L. (V.) braziliensis MHOM/BR/75/M2904

26.07 ± 1.80

38.19 ± 0.04

negative

negative

nt

nt

  1. Abbreviation: nt not tested
  2. aOne replicate negative