Table 1 Primers and PCR amplification conditions
Target gene | Primer name | Primer sequence (5'-3') | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
gltA | Outer-f | GCGATTTTAGAGTGYGGAGATTG | 55 | 1031 | [4] |
Outer-r | TACAATACCGGAGTAAAAGTCAA | ||||
Inner-f | TCATCTCCTGTTGCACGGTGCCC | 60 | 594 | [8] | |
Inner-r | CTCTGAATGAACATGCCCACCCT | ||||
16S rRNA | Forward | GCAAGTCGAACGGACCAAATCTGT | 58 | 1261 | [8] |
Reverse | CCACGATTACTAGCGATTCCGACTTC | ||||
groEL | Forward | TGAAGAGCATCAAACCCGAAG | 55 | 874 | [8] |
Reverse | CTGCTCGTGATGCTATCGG | ||||
msp4 | Forward | GGGTTCTGATATGGCATCTTC | 53 | 656 | This study |
Reverse | GGGAAATGTCCTTATAGGATTCG |