PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis

Parasites & Vectors

Table 1 Primer designed for pvgama gene amplification

Target	Primer sequence (5’ – 3’)^a	MT (°C)	Product size (bp)	aa position
pvgama	Fwd: ATGAAGTGCAACGCCTCC Rev: AAAAATGAATAGGAGCAACG	58	2313	1 to 771
pvgama -Nt	Fwd: ATACGGAATGGAAACAACC Rev: AGTCGGTTCGTTATTCTCG		1284	22 to 449
pvgama -Ct	Fwd: CTGCTCAAGAACACGAAC Rev: GCTTCCACTCTGCAATTC		948	434 to 749
pvgama -CR1	Fwd: GACGATCATCTGTGTTCAAAAA Rev: GACCTCATTTTTGGACTTCTC	60	666	87 to 308
pvgama -VR1	Fwd: GGCGCCTTCCTGCAGTC Rev: CATTAACATGGTGTTGTCGCT		438	330 to 475
pvgama -CR2	Fwd: CAGGCGGCCATCTTACTAA Rev: GCTCCCGTTGACGCCCTT		321	482 to 588
pvgama -VR2	Fwd: GCCGCAAACGCAGACGCC Rev: GTTTGCCGAGAAGCTTCCAC		384	626 to 753

Abbreviations: Nt and Ct amino and carboxyl terminal; CR conserved region, VR variable region; Fwd forward, Rev reverse, MT melting temperature, bp base pair, aa amino acid
^aProtein’s expression start codon was included in forward primer’s 5’ end

ISSN: 1756-3305