Fig. 3
From: Characterising PvRBSA: an exclusive protein from Plasmodium species infecting reticulocytes
Gating strategy for selecting reticulocytes and mature erythrocyte cell population. Doublets were excluded comparing FSC-H to FSC-A cytogram. Cells were then selected by their granularity by plotting SSC-A against FSC-A. The CD45 and CD71 signals were plotted for selecting reticulocyte (CD71 + CD45-) and mature erythrocyte (CD71-CD45-) populations. The cell percentage having bound protein was calculated using the PE signal against SSC-A. A representative histogram from three independent experiments analysing the PE signal for the rPvRBSA binding assay compared with the control is shown