Fig. 1

Primary screening of compounds on Haemonchus contortus. Synthetic pyrazole-5-carboxamides (n = 55) were tested for inhibition of motility of exsheathed third-stage larvae (xL3) of H. contortus at a concentration of 100 μM (after 72 h of exposure). Tolfenpyrad, monepantel and/or moxidectin were also included as control compounds; tolfenpyrad was used as the positive reference-control, as it has known activity against xL3s and L4s of H. contortus [13]. Two active compounds, a-15 and a-17, inhibited xL3 motility by ≥ 70%. Supplementary file 2 shows the “straight” phenotype in larvae exposed to a-15 and a-17, similar to that of the tolfenpyrad control