Skip to main content

Advertisement

Fig. 3 | Parasites & Vectors

Fig. 3

From: Assessing the anthelmintic activity of pyrazole-5-carboxamide derivatives against Haemonchus contortus

Fig. 3

Respiration rates of Haemonchus contortus treated with test or control compounds in vitro. Panels a and b show individual curves of the oxygen consumption rate (OCR) of third larvae (xL3s) (n = 600 per well) following exposure to individual test compounds (a-15 and a-17) and reference controls (tolfenpyrad, tebufenpyrad and monepantel), tested at concentrations of 50 μM and 100 μM, respectively. The OCR data were measured 35 times (2 min-mix 4 min-measure) for 30 min before and 180 min after exposure to each compound using a Seahorse XFe96 flux analyser. Three separate experiments were conducted using 4 replicates in each experiment. Panels c and d show the total oxygen consumption of xL3s (n = 600 per well; calculated from the area under the curve, AUC) following exposure to individual test compounds (a-15 and a-17) and the reference control compounds (tolfenpyrad, tebufenpyrad and monepantel), tested at concentrations of 50 μM and 100 μM, respectively. Variation was expressed as the standard error of the mean (SEM). Significance between values (mean ± SEM) was determined using a nonparametric (Kruskal-Wallis) one-way ANOVA and Dunnett’s multiple comparison test. Asterisks indicate values that are significantly different from one another (**P < 0.01; ****P < 0.0001)

Back to article page