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Table 1 In vitro activity of test compounds. Comparison of activity of compounds a-15 and a-17 with tolfenpyrad and tebufenpyrad on the motility of exsheathed third-stage (xL3) and fourth-stage (L4) larvae of Haemonchus contortus (after 24 h, 48 h and 72 h of exposure) and on the development of L4 (after 7 days of exposure)

From: Assessing the anthelmintic activity of pyrazole-5-carboxamide derivatives against Haemonchus contortus

Compound

Half maximum inhibitory concentration (IC50 μM)

xL3 motility

L4 motility

L4 development

24 h

48 h

72 h

24 h

48 h

72 h

7 days

a-15

55.63 ± 0.18

26.31a

3.97 ± 0.35

a-17

51.60 ± 1.41

15.58a

3.42 ± 0.50

Tolfenpyrad

2.98 ± 0.50

3.85 ± 1.33

3.05 ± 0.47

0.12 ± 0.07

0.06 ± 0.02

0.03 ± 0.02

0.08 ± 0.01

Tebufenpyrad

na

na

na

> 100

> 100

> 100

6.70a

  1. aHalf maximum inhibitory concentration could not be accurately calculated by the log (agonist) versus response - variable slope (four parameter) equation, a IC50 value was estimated
  2. na no activity; “–” indicates where IC50 values were not determined if the log(agonist) vs response - variable slope (four parameters) model could not be used to fit the curve