Fig. 4
Experiment 3: Immediate (acute) inhibition of biting behaviour by a single light pulse tested at different times of the diel night. Inhibition of blood-feeding in mosquitos that were blood-fed in the light versus fed in the dark. The inhibitory effect of light on blood-feeding declined as the dark period progressed. Blood meals were offered during the biological night at ZT12, 14, 16, 18, 20 and 22 under either light or dark conditions. Experimental mosquitoes were subject to a single light pulse while simultaneously being blood-fed. Two-way ANOVA (effect of treatment, F (1,32) = 50.6, P < 0.001; effect of ZT, F (5,32) = 2.2, P = 0.097; interaction, F (5,32) = 1.8, P = 0.161), followed by post-hoc tests (*P <0.05, **P <0.01 and ***P <0.001). Values are mean ± SEM