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Fig. 2 | Parasites & Vectors

Fig. 2

From: NLRP3 inflammasome activation in murine macrophages caused by Neospora caninum infection

Fig. 2

Secretion of inflammasome-mediated IL-1β is induced in mouse peritoneal macrophages infected by N. caninum. a Confirmation of some upregulated genes indicated in the inflammasome PCR array. Peritoneal macrophages (without LPS priming) were stimulated with N. caninum tachyzoites (MOI = 3:1, parasite:cell) for 12 h, and the expression levels of the NLRP3, NLRC4 and NLRC5 genes were measured by qPCR. b-f With or without LPS priming (indicated + or – in the figures), peritoneal macrophages were incubated with N. caninum tachyzoites (MOI = 3:1, parasite:cell) for the indicated time or N. caninum tachyzoites excretory-secretory antigens (ESAs) for 12 h; LPS primed peritoneal macrophages were incubated with ATP (5 mM) for 30 min as positive control. b, d, e Expression of NLRP3, processing of caspase-1 and secretion of IL-β were analyzed by immunoblot. c, f IL-1β production in supernatants was measured by ELISA. Abbreviations: C, control; SN, supernatants; LYS, cell lysates; ESA, excretory secretory antigens. The data are representatives of three independent experiments and presented as the mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001 vs negative control)

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