Fig. 4

NLRP3 inflammasome activation in N. caninum infection. Peritoneal macrophages primed with LPS were incubated with N. caninum tachyzoites (Nc) (MOI = 3:1, parasite:cell) for 10 h or ATP (5 mM) for 30 min. a The localization of NLRP3 and F-actin after stimulation with N. caninum or ATP. b The NLRP3 expression and the secretion of IL-1β after stimulation with N. caninum (10 h) were determined by immunoblot analysis. c The production of IL-1β in the supernatants was measured by ELISA. Abbreviations: C, control; SN, supernatants; LYS, cell lysates; Nc, N. caninum. The data are representative of three independent experiments and presented as the mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001 vs negative control). Scale-bars: 10 μm