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Fig. 8 | Parasites & Vectors

Fig. 8

From: Leishmania LABCG1 and LABCG2 transporters are involved in virulence and oxidative stress: functional linkage with autophagy

Fig. 8

Reduced non-protein thiol levels in L. major lines. a Parasites (3 × 108) were collected from different lines: WT (black histograms), ΔLABCG1-2 (grey histograms), and ΔLABCG1-2 + LABCG1-2 (white histograms) at stationary-phase growth. After three washes with PBS, the cell pellet was resuspended in Tris-HCl 10 mM-EDTA supplemented with 2% Triton X-100 and thiol levels quantified by flow cytometry. b Promastigotes (1 × 109) from different lines were collected, lyophilised and stored at 4 °C until used. The cell pellet was treated as described in Materials and Methods. Data are the mean ± SD from three independent experiments. Significant differences versus WT parasites were determined with the Student’s t-test (*P < 0.01)

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