Symbiont dynamics of the Tibetan tick Haemaphysalis tibetensis (Acari: Ixodidae)

Parasites & Vectors

Table 1 Oligonucleotide primers used for PCR amplification and sequencing

Primer	Species	Target gene	Nucleotide sequence (5′–3′)	Annealing temperature (°C)	Approx. product size (bp)	Reference
CLS-F	Coxiella	16S rRNA	CACGTAGGAATCTACCTTGTAG	55	90	[4]
CLS-R			CGTTTTGTTCCGAAGAAATTAT
Eub27F	Eubacteria	16S rRNA	AGAGTTTGATCCTGGCTCAG	55	1,500	[32]
Eub1492R			TACCTTGTTACGACTT
Rickettsia354F	Rickettsia	16S rRNA	CAGCAATACCGAGTGAGTGATGAAG	56	350	[33]
Rickettsia647R			AGCGTCAGTTGTAGCCCAGATG
Actin-F	Haemaphysalis	actin	CGTTCCTGGGTATGGAATCG	55	100	[4]
Actin-R	Haemaphysalis	actin	TCCACGTCGCACTTCATGAT

ISSN: 1756-3305