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Table 1 Oligonucleotide primers used for PCR amplification and sequencing

From: Symbiont dynamics of the Tibetan tick Haemaphysalis tibetensis (Acari: Ixodidae)

Primer Species Target gene Nucleotide sequence (5′–3′) Annealing temperature (°C) Approx. product size (bp) Reference
CLS-F Coxiella 16S rRNA CACGTAGGAATCTACCTTGTAG 55 90 [4]
CLS-R    CGTTTTGTTCCGAAGAAATTAT    
Eub27F Eubacteria 16S rRNA AGAGTTTGATCCTGGCTCAG 55 1,500 [32]
Eub1492R    TACCTTGTTACGACTT    
Rickettsia354F Rickettsia 16S rRNA CAGCAATACCGAGTGAGTGATGAAG 56 350 [33]
Rickettsia647R    AGCGTCAGTTGTAGCCCAGATG    
Actin-F Haemaphysalis actin CGTTCCTGGGTATGGAATCG 55 100 [4]
Actin-R Haemaphysalis actin TCCACGTCGCACTTCATGAT