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Fig. 3 | Parasites & Vectors

Fig. 3

From: Immunolocation and enzyme activity analysis of Cryptosporidium parvum enolase

Fig. 3

Polyclonal antibody detection of rCpEno immune serum. Rabbit polyclonal antisera were collected after the third immunisation. The reactivity and specificity of antibodies were tested by ELISA (a) and Western blot (b). A series of diluted antibodies were examined for their specific binding activity to either rCpEno or rabbit muscle enolase (negative control). A good antibody titer was detected when the dilution multiple was higher than 1/102,400, and a 52.5 kDa specific band was observed by western blot assay. Data shown are the mean values ± standard deviations

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