Fig. 4
From: Identification and validation of a Schistosoma japonicum U6 promoter
sjU6 promoter is active in HEK293 cells. HEK293 cells were transfected with 2ug of pHBLV-sgRNA970 plasmid and the transcript of the 98 bp sgRNA970 was detected by RT-PCR after 48 h. Lane M: Trans2K DNA maker; Lane 1: unstransfected HEK293 cell cDNA; Lane 2: transfected HEK293 cell RNA treated with DNAseI, but without reverse transcription; Lane 3: cDNA synthesized from transfected HEK293 cell RNA treated with DNAseI