Fig. 5
From: Identification and validation of a Schistosoma japonicum U6 promoter
Lentivrius induced Cas9-ZsGreen expression in S. japonicum. Schistosomula were transduced with pHBLV- CMVIE-Cas9-ZsGreen lentivirus. a Fluorescence microscopic detection of the ZsGreen fluorescent signal in lentivirus-transduced schistosomula. HEK293 cells transduced with lentivirus served as a positive control. Schistosomula without lentivirus transduction served as a negative control. b Western blot detection of Cas9-ZsGreen fusion protein expression. Lane 1: schistosomula without lentivirus transduction; Lane 2: HEK293 cells transduced with lentivirus as positive control; Lane 3: lysed virus as negative control; Lane 4: schistosomula transduced with lentivirus