Fig. 6

sjU6 promoter is active in S. japonicum. Schistosomula were transduced 14 dpi with pHBLV-sgRNA970 lentivirus for 48 h and cultured for additional 48 h after viral exposure. Lane M: DL2000 DNA marker; Lane 1: amplification of the 445 bp sjU6-sgRNA970 expression cassette from gDNA of untrasduced schistosomula; Lane 2: amplification of the 445 bp sjU6-sgRNA970 expression cassette from transduced schistosomula gDNA; Lane3: amplification of the 98 bp sgRNA970 from untransduced schistosomula cDNA; Lane4: amplification of the 98 bp sgRNA970 from transduced schistosomula RNA treated with DNAseI, but without reverse transcription; Lane 5: amplification of the PSMD internal control gene from cDNA synthesized from transduced schistosomula RNA treated with DNAseI; Lane 6, amplification of the 98 bp sgRNA970 from cDNA synthesized from transduced schistosomula RNA treated with DNAseI; Lane 7: amplification of the 445 bp sjU6-sgRNA970 expression cassette from cDNA synthesized from transduced schistosomula RNA treated with DNAseI