Fig. 2

Silencing of the SsGST-mu1 gene in the scabies mite S. scabiei by RNAi. The transcription of the SsGST-mu1 gene was reduced by 35.14% (t = 4.509, P = 0.0020) and 34.63% (t = 3.45, P = 0.0110) in mites treated with SsGST-mu1 dsRNA. 24 h dsRNA incubation was followed by 24 h (a) or 48 h (b) serum incubation. The mean SsGST-mu1 gene transcription normalised to SsEF1α gene transcription was determined by qPCR. Depicted is the calculated copy number per μl of cDNA (generated from 250 ng of total RNA template) in preparations from mites that were immersed in SsGST-mu1 dsRNA (treatment) or LacZ dsRNA (control). N = 5 × 30 mites for each group (treatment or control). Error bars indicate mean ± SEM