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Table 2 Exposure of S. scabiei mites to dsRNA under different experimental conditions

From: Gene silencing by RNA interference in Sarcoptes scabiei: a molecular tool to identify novel therapeutic targets

Experiment

Incubation

Survival rate (%)

dsRNA

75% serum

Normal saline

Out of liquida

1

24 h, 4 °C

na

na

na

100

2

48 h, 4 °C

na

na

na

100

3

72 h, 4 °C

na

na

na

100

4

24 h, 4 °C

24 h, RT

na

na

93

5

24 h, 4 °C

48 h, RTb

na

na

100

6

24 h, 4 °C

na

24 h, RT

na

100

7

24 h, 4 °C

24 h, RT

24 h, RT

na

100

8

24 h, 4 °C

na

na

48 h, RTa

26

  1. Abbreviations: na, not applicable; RT, room temperature
  2. adsRNA was removed and mites were kept in humidifier chamber
  3. bHeat-inactivated serum was replaced after first 24 h