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Table 2 Exposure of S. scabiei mites to dsRNA under different experimental conditions

From: Gene silencing by RNA interference in Sarcoptes scabiei: a molecular tool to identify novel therapeutic targets

Experiment Incubation Survival rate (%)
dsRNA 75% serum Normal saline Out of liquida
1 24 h, 4 °C na na na 100
2 48 h, 4 °C na na na 100
3 72 h, 4 °C na na na 100
4 24 h, 4 °C 24 h, RT na na 93
5 24 h, 4 °C 48 h, RTb na na 100
6 24 h, 4 °C na 24 h, RT na 100
7 24 h, 4 °C 24 h, RT 24 h, RT na 100
8 24 h, 4 °C na na 48 h, RTa 26
  1. Abbreviations: na, not applicable; RT, room temperature
  2. adsRNA was removed and mites were kept in humidifier chamber
  3. bHeat-inactivated serum was replaced after first 24 h