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Fig. 1 | Parasites & Vectors

Fig. 1

From: Clonorchis sinensis lysophospholipase A upregulates IL-25 expression in macrophages as a potential pathway to liver fibrosis

Fig. 1

IL-25 is highly expressed in CsLysoPLA-stimulated RAW264.7 cells. a Quantitative real-time PCR analysis of IL-25 in RAW264.7 cells treated with CsLysoPLA (1, 5, 10 and 20 μg/ml) and PBS (0 μg/ml, negative control) for 12, 24 and 48 h. CsFBPase (20 μg/ml) and MSA (20 μg/ml) were used as control proteins. Data are shown as mean ± SEM. b Quantitative real-time PCR analysis of TNF-α, iNOS, IL-6, IL-13, IL-10 and IL-33 in RAW264.7 cells treated with CsLysoPLA (1, 5, 10 and 20 μg/ml) and PBS (0 μg/ml, negative control) for 24 h. c Western blot analysis of IL-25 in RAW264.7 cells treated with CsLysoPLA (10 μg/ml) and the equal volume of PBS for 24 h. GADPH was used as a loading control. d Quantification of western blot data in Fig. 1c. Data are shown as mean ± SEM. ** P < 0.01, ***P < 0.001. e Immunofluorescence staining analysis of IL-25 (green) in RAW264.7 cells treated with CsLysoPLA (10 μg/ml) and the equal volume of PBS for 24 h. Nuclei were stained with DAPI (blue). Original magnification ×100. Scale-bars: 200 μm. f Quantification of immunofluorescence data in Fig. 1e. Data are shown as mean ± SEM. ***P < 0.001

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