Clonorchis sinensis lysophospholipase A upregulates IL-25 expression in macrophages as a potential pathway to liver fibrosis

Table 1 Primer sequences for quantitative real-time PCR

Gene	Sequence (5′-3′)	Accession number
α-SMA	Forward: CCAGGGCTGTTTTCCCATCC	NM_001613.2
α-SMA	Reverse: GCTCTGTGCTTCGTCACCCA
IL-25	Forward: TCTACCGAGTCTCCTTGGCT	NM_080729.3
IL-25	Reverse: ATTGTACACCTGGCCCTCTC
TNF-α	Forward: GACAGTGACCTGGACTGTGG	NM_013693.3
TNF-α	Reverse: TGAGACAGAGGCAACCTGAC
iNOS	Forward: ACCTTGTTCAGCTACGCCTT	NM_010927.3
iNOS	Reverse: CATTCCCAAATGTGCTTGTC
IL-6	Forward: AGTCCGGAGAGGAGACTTCA	NM_031168.1
IL-6	Reverse: ATTTCCACGATTTCCCAGAG
PKA	Forward: CCTGTTCCCACCCTATCACT	NM_001277898.1
PKA	Reverse: TGGAAGCCATCACTCAGTCT
B-Raf	Forward: TCCACGTTGGCATTGTTAGT	XM_006505358.2
B-Raf	Reverse: TCACTCCTGTAAGCGTCCTG
ERK1	Forward: TCCCAGGAGGACCTTAATTG	NM_011952.2
ERK1	Reverse: AAGGTTAACATCCGGTCCAG
ERK2	Forward: TGAGGATGTTAGGCTTCGTCT	NM_001038663.1
ERK2	Reverse: AAAGTCCACTCCCACAATGC
β-actin^a	Forward: TGGACTTCGAGCAAGAGATG	NM_001101.3
β-actin^a	Reverse: GAAGGAAGGCTGGAAGAGTG
β-actin^b	Forward: GGAATGGGTCAGAAGGACTC	NM_007393.5
β-actin^b	Reverse: CATGTCGTCCCAGTTGGTAA

ISSN: 1756-3305