Fig. 2

Cytokine production in supernatants of splenocyte cultures stimulated with anti-CD3 and anti-CD28 monoclonal antibodies at 10 μg/ml and 2 μg/ml, respectively. Splenocytes were harvested from Anisakis pegreffii or Pseudoterranova decipiens infected BALB/c mice at week 12 post - infection. Green histogram: mice orally infected with 2 A. pegreffii L3 (L3 p.o.); red histogram: mice intraperitoneally infected with 2 A. pegreffii L3 or 2 P. decipiens L3 (L3 p.i.); blue histograms: mice orally inoculated with A. pegreffii or P. decipiens crude worm extracts (CWE); open histogram: mice intraperitoneally inoculated with phosphate buffered saline (PBS). Significances (*P < 0.05) were calculated between experimental and PBS control groups. Each experiment was conducted in triplicate