Fig. 3

Cytokine production in supernatants of splenocyte cultures stimulated with Anisakis pegreffii or Pseudoterranova decipiens crude worm antigens (CWE). Splenocytes from BALB/c mice were harvested at week 12. Green histogram: mice orally infected with 2 A. pegreffii L3 (L3 p.o.); red histogram: mice intraperitoneally infected with 2 A. pegreffii L3 or 2 P. decipiens L3 (L3 p.i.); blue histograms: mice orally inoculated with A. pegreffii CWE or P. decipiens CWE (CWE); open histogram: mice intraperitoneally inoculated with phosphate buffered saline (PBS). Abbreviations: No Ag, non-stimulated splenocytes; Ag, splenocytes stimulated with specific CWE. Significances (*P < 0.05) were calculated between experimental and PBS control groups. Each experiment was conducted in triplicate