Molecular investigation and phylogeny of Anaplasmataceae species infecting domestic animals and ticks in Corsica, France

Table 2 Primers and probes used in this study

Targeted microorganisms	Targeted gene	Primers and probe^a	Sequences 5′-3′	Annealing temperature (°C)	References
		qPCR
Anaplasmataceae	23S rRNA	TtAna-F TtAna-R TtAna-S^a	TGACAGCGTACCTTTTGCAT GTAACAGGTTCGGTCCTCCA FAM-CTTGGTTTCGGGTCTAATCC-TAMRA	60	[28, 29]
		Conventional PCR
Anaplasmataceae	23S rRNA	Ana23S-212f Ana23S-753r	ATAAGCTGCGGGGAGTTGTC TGCAAAAGGTACGCTGTCAC	55	[28, 29]
Anaplasma spp.	rpoB	Ana-rpoBF Ana-rpoBR	GCTGTTCCTAGGCTYTCTTACGCGA AATCRAGCCAVGAGCCCCTRTAWGG	55	[27]
Ehrlichia spp.	groEL	Ehr-groEL-F Ehr-groEL-R	GTTGAAAARACTGATGGTATGCA ACACGRTCTTTACGYTCYTTAAC	50	[27]
Ticks	12S rRNA	T1B T2A	AAACTAGGATTAGATACCCT AATGAGAGCGACGGGCGATGT	51	[26]

ISSN: 1756-3305