A review of Sarcoptes scabiei : past, present and future

Larry G. Arlian; Marjorie S. Morgan

doi:10.1186/s13071-017-2234-1

Parasites & Vectors

Table 3 Characteristics of published ELISAs using crude mite extract as the target antigen to detect antibodies circulating in serum of scabietic hosts

From: A review of Sarcoptes scabiei: past, present and future

Target antigen		Host species	Detecting antibodies		Sensitivity (%)	Specificity (%)	Reference
Mite variety	Extract		Primary	Conjugate
canis (on Rb)	Aq Hmgt	rabbit (canis)		HRP-Go × Rb Ig			[116]
canis (on Rb)	Aq Hmgt	rabbit (canis)		HRP-Go × Rb Ig			[90]
canis (on Rb)	Aq Hmgt	rabbit (canis)		HRP-Go × Rb IgM			[90]
canis (on Rb)	Aq Hmgt	dog		HRP-Go × Dog Ig			[117]
canis (on Rb)	Aq Hmgt	dog		HRP-Go × Dog IgM			[117]
canis (on Rb)	Aq Hmgt	dog		HRP-Go × Dog IgG1			[117]
canis (on Rb)	Aq Hmgt	dog		HRP-Go × Dog IgG2			[117]
canis (on Rb)	Aq Hmgt	human		HRP-Go × Hu Ig			[127]
canis (on Rb)	Aq Hmgt	human	BT-Go × Hu Ig	HRP-SA	45		[87]
canis (on Rb)	Aq Hmgt	human	BT-Mu × Hu IgD	HRP-SA	0		[87]
canis (on Rb)	Aq Hmgt	human	BT-Mu × Hu IgE	HRP-SA	2		[87]
canis (on Rb)	Aq Hmgt	human	BT-Go × Hu IgG	HRP-SA	28		[87]
canis (on Rb)	Aq Hmgt	human	BT-Go × Hu IgM	HRP-SA	74		[87]
canis		ibex		HRP-Dnky × Go IgG	33	89	[128]
caprae	PBS Hmgt	goat		HRP-Dnky × Go IgG			[118]
caprae	PBS Hmgt	sheep	Mu × Sh IgG	HRP-SA	90		[129]
cuniculi	PBS Hmgt	mice		HRP-Go × Mu			[126]
ovis	PBS Hmgt	sheep	BT-Mu × Sh IgG; BT-Go × Mu IgG	HRP-SA	88	96	[102]
suis		pig			100		[96]
suis	Aq Ext	pig		HRP-Rb × Pig Ig	78	97	[130]
suis	Aq Ext	pig		HRP-Rb × Pig IgG-Fc	54	94	[98]
suis	Aq Ext	pig		HRP-Rb × Pig IgG-Fc	92	99	[98]
suis	Aq Ext	pig		HRP-Rb × Pig?	73	81	[131]
suis	Aq Ext	pig¹		HRP-Rb × Pig?	71	77	[131]
suis		pig			89		[97]
suis	PBS Hmgt	pig		HRP-Rb x Pig IgG	100	100	[132]
suis		wild boar			75	80	[133]
suis		ibex	BT-rProtein G	HRP-avidin	93	94	[128]
vulpes	Aq Ext	red fox²	Mu x Dog IgG	HRP-Rb × Mu Ig	95	~100	[103]
vulpes	Aq Ext	red fox	Mu x Dog IgG	HRP-Rb × Mu Ig	95	83	[134]
vulpes	Aq Ext	red fox²	Mu x Dog IgG	HRP-Rb × Mu Ig	95–100		[135]
vulpes	Aq Ext	wolf	Mu x Dog IgG	HRP-Rb × Mu Ig			[136]
vulpes		dog		AP-Go × Dog IgG	84	90	[137]
vulpes	PBS Hmgt	dog		HRP-Mu × Dog IgG			[104]
vulpes	PBS Hmgt	dog		HRP-Mu × Dog IgG	83	92	[138]
vulpes	Aq sonicate	chamois	BT-rProtein G	HRP-avidin	93	97	[99]
vulpes	Aq sonicate	ibex	BT-rProtein G	HRP-avidin			[139]
vulpes	PBS Hmgt	pig		HRP-Rb × Pig Ig			[140]
vulpes	PBS Hmgt	pig		HRP-Go × Pig Ig	50–80	≥ 98	[95]
vulpes	PBS Hmgt	pig			30		[97]
vulpes	PBS Hmgt	pig			53		[97]
vulpes	PBS Hmgt	pig			70		[97]
vulpes	PBS Hmgt	pig			0–100		[96]
vulpes	PBS Hmgt	pig			100		[96]
vulpes	PBS Hmgt	human		HRP-Go × Hu IgG	48	100	[105]

All ELISAs tested serum except pig¹ that tested meat juice and red fox² that tested body fluids
Abbreviations: AP alkaline phosphatase, Aq Aqueous (presumably water), BT biotinylated, Dnky donkey, Ext extract, Go goat, Hmgt homogenate, HRP horseradish peroxidase, Hu human, Mu mouse, PBS phosphate buffered saline, Rb rabbit, SA streptavidin, Sh sheep, x = anti-;? = not reported

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