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Table 3 Characteristics of published ELISAs using crude mite extract as the target antigen to detect antibodies circulating in serum of scabietic hosts

From: A review of Sarcoptes scabiei: past, present and future

Target antigen Host species Detecting antibodies Sensitivity (%) Specificity (%) Reference
Mite variety Extract   Primary Conjugate    
canis (on Rb) Aq Hmgt rabbit (canis)   HRP-Go × Rb Ig    [116]
canis (on Rb) Aq Hmgt rabbit (canis)   HRP-Go × Rb Ig    [90]
canis (on Rb) Aq Hmgt rabbit (canis)   HRP-Go × Rb IgM    [90]
canis (on Rb) Aq Hmgt dog   HRP-Go × Dog Ig    [117]
canis (on Rb) Aq Hmgt dog   HRP-Go × Dog IgM    [117]
canis (on Rb) Aq Hmgt dog   HRP-Go × Dog IgG1    [117]
canis (on Rb) Aq Hmgt dog   HRP-Go × Dog IgG2    [117]
canis (on Rb) Aq Hmgt human   HRP-Go × Hu Ig    [127]
canis (on Rb) Aq Hmgt human BT-Go × Hu Ig HRP-SA 45   [87]
canis (on Rb) Aq Hmgt human BT-Mu × Hu IgD HRP-SA 0   [87]
canis (on Rb) Aq Hmgt human BT-Mu × Hu IgE HRP-SA 2   [87]
canis (on Rb) Aq Hmgt human BT-Go × Hu IgG HRP-SA 28   [87]
canis (on Rb) Aq Hmgt human BT-Go × Hu IgM HRP-SA 74   [87]
canis   ibex   HRP-Dnky × Go IgG 33 89 [128]
caprae PBS Hmgt goat   HRP-Dnky × Go IgG    [118]
caprae PBS Hmgt sheep Mu × Sh IgG HRP-SA 90   [129]
cuniculi PBS Hmgt mice   HRP-Go × Mu    [126]
ovis PBS Hmgt sheep BT-Mu × Sh IgG; BT-Go × Mu IgG HRP-SA 88 96 [102]
suis   pig    100   [96]
suis Aq Ext pig   HRP-Rb × Pig Ig 78 97 [130]
suis Aq Ext pig   HRP-Rb × Pig IgG-Fc 54 94 [98]
suis Aq Ext pig   HRP-Rb × Pig IgG-Fc 92 99 [98]
suis Aq Ext pig   HRP-Rb × Pig? 73 81 [131]
suis Aq Ext pig1   HRP-Rb × Pig? 71 77 [131]
suis   pig    89   [97]
suis PBS Hmgt pig   HRP-Rb x Pig IgG 100 100 [132]
suis   wild boar    75 80 [133]
suis   ibex BT-rProtein G HRP-avidin 93 94 [128]
vulpes Aq Ext red fox2 Mu x Dog IgG HRP-Rb × Mu Ig 95 ~100 [103]
vulpes Aq Ext red fox Mu x Dog IgG HRP-Rb × Mu Ig 95 83 [134]
vulpes Aq Ext red fox2 Mu x Dog IgG HRP-Rb × Mu Ig 95–100   [135]
vulpes Aq Ext wolf Mu x Dog IgG HRP-Rb × Mu Ig    [136]
vulpes   dog   AP-Go × Dog IgG 84 90 [137]
vulpes PBS Hmgt dog   HRP-Mu × Dog IgG    [104]
vulpes PBS Hmgt dog   HRP-Mu × Dog IgG 83 92 [138]
vulpes Aq sonicate chamois BT-rProtein G HRP-avidin 93 97 [99]
vulpes Aq sonicate ibex BT-rProtein G HRP-avidin    [139]
vulpes PBS Hmgt pig   HRP-Rb × Pig Ig    [140]
vulpes PBS Hmgt pig   HRP-Go × Pig Ig 50–80 ≥ 98 [95]
vulpes PBS Hmgt pig    30   [97]
vulpes PBS Hmgt pig    53   [97]
vulpes PBS Hmgt pig    70   [97]
vulpes PBS Hmgt pig    0–100   [96]
vulpes PBS Hmgt pig    100   [96]
vulpes PBS Hmgt human   HRP-Go × Hu IgG 48 100 [105]
  1. All ELISAs tested serum except pig1 that tested meat juice and red fox2 that tested body fluids
  2. Abbreviations: AP alkaline phosphatase, Aq Aqueous (presumably water), BT biotinylated, Dnky donkey, Ext extract, Go goat, Hmgt homogenate, HRP horseradish peroxidase, Hu human, Mu mouse, PBS phosphate buffered saline, Rb rabbit, SA streptavidin, Sh sheep, x = anti-;? = not reported
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