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Fig. 1 | Parasites & Vectors

Fig. 1

From: Arginine kinase from Haemonchus contortus decreased the proliferation and increased the apoptosis of goat PBMCs in vitro

Fig. 1

Expression, purification and Western blot analysis of rHc-AK. Lane M: standard protein molecular weight marker. a Expression of rHc-AK was induced with 1 mM IPTG. Lane 0: recombinant expression vector before induction; Lanes 1–7: protein expression at different time points. b Lane 1: purification of recombinant protein. c Lane 1: purified rHc-AK was electrophoresed and transferred to a membrane for Western blot analysis with rat anti-rHc-AK sera. Lane 2: membrane incubated with normal rat sera (as control)

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