Fig. 4

Cytokine production after treatment with ADO receptor antagonist in mice infested with ticks. BALB/c mice (6/group) were three times tick-infested and treated daily, throughout all infestations with a non-selective antagonist of ADO (8-pSPT, 20 mg/kg i.p.) or saline. Mice were killed on the 3rd and 7th day (3D and 7D) of the third infestation and their lymph nodes cells were cultured with Con-A (2 mg/ml). After 24 h of incubation, the supernatants were collected and assayed for IL-2 (a), IL-4 (b), IL-6 (c), Il-17 (d), INFγ (e), TNF (f) and IL-10 (g) cytokines by CBA. Data are presented as the mean of cytokine production ± SEM from two independent experiments