Fig. 2

Genotype confirmation and GFP expression profile of clone 21 (C-21) and 56 (C-56) of LplA1-cKO transgenic parasites. a Genotype of cloned transgenic parasites were analyzed by PCR using the primer pairs gfp-73/4–1053 (Lane 1) to detect a 1.6 kb fragment crossing parasite genome and the vector, LplA1–10/LplA1–11 (Lane 2) to amplify a 2.5 kb fragment crossing lplA1 locus in WT parasite, and 4–1885/4–5083 (Lane 3) to amplify a 0.99 kb fragment of the plasmid vector or episome (also see legend to Fig. 1). The fragments were verified by sequencing. b Flow cytometric profiles of gfp expression by cloned LplA1-cKO or control transgenic parasites with or without exposure in vivo to ATc. c Mean GFP fluorescence intensity of cloned LplA1-cKO parasites. Mean (± SD) from triplicate flow cytometric analyses are presented, ***P < 0.001 (Student’s t-test). d Fluorescence photomicrographs showing gfp expression by transgenic parasites with or without ATc exposure in vivo. Scale-bars: 5 μm