Evaluation of immune protection induced by DNA vaccines from Haemaphysalis longicornis paramyosin in rabbits

Parasites & Vectors

Table 1 Data from rabbits tests evaluating Pmy DNA for efficacy as anti-H. longicornis vaccine

Group	No. of animals	Total no. of ticks	Total no. of biting ticks	Mortality (%)	Feeding time (day)	Engorgement weight/female (mg)	Ovipositions/female (mg)	Hatchability (%)	NET	EWPF	H	Eff (%)
Control	6	240	202	15.63 ± 0.71	7.44 ± 0.08	170.20 ± 4.00	78.77 ± 4.96	79.93 ± 4.50	–	–	–	–
pcDNA3.1(+)-Pmy	6	240	174	27.31 ± 0.46^*	7.50 ± 0.06	109.61 ± 4.24^*	48.39 ± 4.06^*	76.02 ± 3.58	0.86	0.61	0.95	50

NET = Reduction in tick numbers = Total number of biting ticks from the pcDNA3.1(+)-Pmy group / Total number of biting ticks from the control group
EWPF = Reduction in weight of eggs per female = The average eggs weight of female adult from the pcDNA3.1(+)-Pmy group/ The average eggs weight of female adult from the control group
H = Reduction in hatchability = Hatchability from pcDNA3.1(+)-Pmy group / Hatchability from control group
Eff = Overall efficacy compared to control = 100 [1-(NET × EWPF × H)]
^* P < 0.0001

ISSN: 1756-3305