Fig. 1

The frequencies of B10 cells post-infection and the induction of B10 cells by EgPSC-ESPs in vitro. a Representative frequencies of B10 cell subsets among total splenic CD19⁺ B cells in control and infected mice. Splenic cell suspensions were prepared and cultivated for 6 h. The percentages of IL-10⁺, CD1d^highCD5⁺IL-10⁺, CD1d^highCD5⁻IL-10⁺ among the CD19⁺ B cells were analyzed by flow cytometry. b Ratio of B10 cell subsets among the total splenic CD19⁺ B cells in control mice (n = 15) and infected mice (n = 15). Differences were analyzed by t-test. c Changes in serum IL-10 levels of control and infected mice, determined by CBA (BD Bioscience). Differences were analyzed by the Mann-Whitney U-test. d Representative flow cytometry plots of B10 cells and their subsets post-stimulation with PBS, LPS, and EgPSC-ESPs. e Induction of B10 cells and their subsets stimulated by PBS, LPS and EgPSC-ESPs. Differences were analyzed by one-way ANOVA. f The relative expression of IL-10 in cultured B cells stimulated by PBS, LPS and EgPSC-ESPs using quantitative real-time PCR. Differences were analyzed by the Kruskal-Wallis H-test. All data are expressed as means ± SD of triplicate wells in three independent experiments. Asterisks indicate statistically significant differences among the three groups. *P < 0. 05; **P < 0.01