Fig. 2

The frequencies of Th17 cells post-infection and the inhibitory effects of EgPSC-ESPs on the differentiation of Th17 cells from naïve CD4⁺ T cells under Th17-polarzing conditions. a Representative plots of splenic IL-17⁺CD4⁺ T cells in control and infected mice. b Percentages of Th17 cells among the total splenic CD4⁺ T cells in control mice (n = 15) and infected mice (n = 15). c Serum levels of IL-17A, IL-6, IFN-γ and TNF-α in control and infected mice determined by CBA (BD Bioscience). d Cytokine profiles in the culture supernatants of naïve CD4⁺ T cells after EgPSC-ESPs treatment under Th17-polarizing conditions. Differences among groups were analyzed by Student’s t-test. All data represent the means ± SD. Asterisks indicate statistically significant differences among the three groups. *P < 0. 05; **P < 0.01