Fig. 3

The frequencies of B17 cells post-infection and the immune-phenotype of B17 cells. Single-cell suspensions from the spleens of EgPSCs-infected and control mice were stimulated to analyze the percentages of IL-17A⁺ cells among the splenic CD19⁺ B cells by flow cytometry. The CD5 and CD1d markers that define B10 cells were used to determine the phenotype of B17 cells. a Example of the gating strategy shown on representative dot-plots of stimulated splenic IL-17A⁺ cells and their subsets. CD19⁺ B lymphocytes were gated, followed by the sub-gating of CD1d⁺ and CD5⁺ cells to determine the subpopulations that secrete most of IL-17A in B cells. Alternatively, IL17⁺CD19⁺ B cells were gated directly to compare the dynamic changes pre- and post-infection. b Frequencies of IL17A⁺, CD1d^highIL-17A⁺, CD1d^lowIL17A⁺, CD5⁺IL17A⁺, CD5⁻IL-17A⁺, CD1d^highCD5⁺IL-17A⁺, CD1d^highCD5⁻IL-17A⁺, CD1d^lowCD5⁺IL-17A⁺ and CD1d^lowCD5⁻IL-17A⁺ cells among splenic CD19⁺ B cells in control and infected mice. c The average percentages of IL-17A⁺ producing B cell subsets among the total splenic CD19⁺ B cells in control and infected mice. Data are expressed as means ± SD (n = 15 mice). Differences among groups were analyzed by Student’s t-test. Asterisks indicate statistically significant differences between the control and infected group. *P < 0. 05; **P < 0.01