Fig. 4

The inhibitory effects of EgPSC-ESPs on the induction of B17 cells in vitro. Splenic CD19⁺ B cells were sorted from normal BALB/c mice using a mouse CD19⁺ B cell isolation kit (Miltenyi, Germany) and cultured for 72 h in the presence of PBS, 10 μg/ml LPS, or 10 μg/ml EgPSC-ESPs. The single-cells suspensions were then collected to analyze the percentages of IL-17A⁺ and CD1d^highCD5⁻IL-17A⁺, CD1d^highCD5⁺IL-17A⁺ cells in CD19⁺B cells by flow cytometry. a Representative plots of B17 cells and their subsets after stimulation of PBS, LPS and EgPSC-ESPs. b Statistical analysis results. c Relative expression of IL-17A in cultured B cells stimulated by PBS, LPS and EgPSC-ESPs using quantitative real-time PCR. Differences were analyzed by one-way ANOVA. All data are expressed as means ± SD of triplicate wells in three independent experiments. Asterisks indicate statistically significant differences among the three groups. *P < 0. 05; **P < 0.01