Fig. 1

Expression of the 14-3-3 isoforms in C6/36 HT and Aag-2 cells. a, b The mRNA expression of 14-3-3ε and 14-3-3ζ. RT-PCR was performed to evaluate the presence of 14-3-3 mRNA transcript from C6/36 HT and Aag-2 cells, confirmed that both cell lines expressed 14-3-3ε and 14-3-3ζ isoforms at the mRNA level. S7 mRNA was used as an internal control. c 14-3-3 protein expression in C6/36 HT and Aag-2 cells. Cell lysates were assessed for the presence of 14-3-3 proteins by Western blot, which demonstrated two distinctive bands with the predicted sizes of 30 and 29 kDa corresponding to 14-3-3Ɛ and 14-3-3ζ protein isoforms, respectively in C6/36 HT and Aag-2 cells. d Intracellular distribution of 14-3-3 proteins in C6/36 HT cells. The immunofluorescent staining of 14-3-3 proteins reveals the presence of the 14-3-3 proteins (red) in the cytoplasm of C6/36 HT cells. Nuclei were stained with DAPI (blue). Scale-bars: 10 μm