Fig. 3

Effect of the 14-3-3 inhibitor on the phagocytosis of S. typhimurium-GFP and FITC-labeled E. coli in C6/36 HT cells. C6/36 HT cells pre-treated with 14-3-3 inhibitor at various concentrations were evaluated for phagocytosis of S. typhimurium-GFP (a) and FITC-labeled E. coli (b). Phagocytosis of both S. typhimurium and E. coli was significantly decreased by the treatment with 14-3-3 inhibitors in a dose-dependent manner. DMSO and medium alone, as well as phagocytosis performed at 4 °C, were used as negative control. Experiments were performed in triplicate, and data from three experiments are presented as the mean ± standard error, SE