Fig. 4

Inhibition of E. coli -containing phagosome formation by the 14-3-3 inhibitor in C6/36 HT cells. C6/36 HT cells were treated with 14-3-3 inhibitor in different concentrations and evaluated for the ability to form phagosome with pHrodo green E. coli bacteria. Phagosome formation (a, b) was significantly reduced by the treatment with inhibitors in dose-dependent manner (c) Representative histograms of phagosome formation by untreated C3/36 HT cells on ice (left, negative control, 4 °C), treated with 12.5 μM of 14-3-3 inhibitor (middle), and untreated C6/36 HT cells at 34 °C, in medium (right, positive control). Experiments were performed in triplicate and represented as the mean ± standard error, SE. Representative data from three independent experiments are shown