Table 1 Templates for DsiRNA generation designed for 14-3-3 silencing. DsiRNAs were synthesised using the custom Dicer-substrate siRNA Construction Kit (IDT, USA). RNA duplex has an asymmetric design with a single 2-base 3′-overhang on the antisense strand and is blunt on the other end; the blunt end is modified with DNA bases marked in bold
Sequence (5′-3′) | |
|---|---|
DsiRNA14-3-3ε | |
DsiRNA duplex1For | CAGUCUUCUACUACGAGAUCCUAAA |
DsiRNA duplex1Rev | UUUAGGAUCUCGUAGUAGAAGACUGAG |
DsiRNA duplex2For | GGGAGAUUACUACAGAUACCUAGCC |
DsiRNA duplex2Rev | GGCUAGGUAUCUGUAGUAAUCUCCCUU |
DsiRNA duplex3For | GGGAAAUCUGCUACGAAGUAUUGGG |
DsiRNA duplex3Rev | CCCAAUACUUCGUAGCAGAUUUCCCUA |
DsRNA14-3-3ζ | |
DsiRNA duplex1For | GGUCAACAAGACAUAGCAAUUGGAA |
DsiRNA duplex1Rev | UUCCAAUUGCUAUGUCUUGUUGACCUC |
DsiRNA duplex2For | CAGUGACAUCGCAAUGACUGACCTT |
DsiRNA duplex2Rev | AAGGUCAGUCAUUGCGAUGUCACUGGC |
DsiRNA duplex3For | UCAGCUGAGUGGUGUAUAAAGAGAA |
DsiRNA duplex3Rev | UUCUCUUUAUACACCACUCAGCUGAUU |
DsRNANeg | |
DsiRNANegFor | Phos-CUUCCUCUCUUUCUCUCCCUUGUGA |
DsiRNANegRev | UCACAAGGGAGAGAAAGAGGAAGGA |