Fig. 4
IL-32 amplifies the production of inflammatory cytokines induced by L. braziliensis during murine ear dermis infection. Lesion-draining submandibular lymph node cells were stimulated with specific antigens from L. amazonensis or L. braziliensis for 24 h (TNF-α) or 72 h (IFN-γ and IL-10). The production of (a) IFN-γ, (b) TNF-α and (c) IL-10 was evaluated by ELISA in culture supernatants. The results represent the mean ± standard deviation of 8 animals per group. *P < 0.05, (WT × IL-32γTg, Student’s t-test)