Fig. 5

a Chemokine and cytokine production in uninfected (medium), A. phagocytophilum-infected, IFN-γ-stimulated, A. phagocytophilum-infected + INF-γ-stimulated, and LPS-stimulated (200 ng/ml) wild-type Hoxb8 neutrophils at different time points. MIP-1α, RANTES, TNF and IL-6 were measured in the supernatants using CBA assay. Mean and SD from 4 independent experiments are shown. Differences between infected and/or stimulated cells and the medium controls at each time point were analysed using the two-tailed Mann-Whitney test. *P < 0.05. b RANTES production of A. phagocytophilum-infected, and A. phagocytophilum-infected + IFN-γ-stimulated wild-type (WT), IFN-γ R^−/− and iNOS^−/− Hoxb8 neutrophils. RANTES was measured in the supernatants using CBA assay. Mean and SD from 4 independent experiments are shown. Differences between infected and infected + IFN-γ-stimulated cells at each time point were analysed using the two-tailed Mann-Whitney test. *P < 0.05