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Fig. 6 | Parasites & Vectors

Fig. 6

From: The substructure of three repetitive DNA regions of Schistosoma haematobium group species as a potential marker for species recognition and interbreeding detection

Fig. 6

Reverse line blot (RLB) analysis. a Biotinylated PCR products targeting S. haematobium (Lane 1) and S. bovis (Lane 2) genomic DNA showing species specific PCR amplicons by agarose gel electrophoresis and their corresponding RLB analysis; Lane 3: negative control reaction without DNA. b The amplicons indicated by arrows in Fig. 5 (Lanes 5, 10, 13, 14 and 15) are shown in Lanes 1–5 of this figure. The “strip design” shows the covalent binding sites of the designed S. haematobium and S. bovis specific oligonucleotides to the Biodyn C membranes. About 15 μl of the amplified PCR products were used to hybridize with the indicated specific oligonucleotides. Strip number 6 represents a negative control

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